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This volume presents essential information on the detection of bacterial and phytoplasmal plant pathogens and the diagnosis of the diseases caused by them. It includes a wide range of protocols that enable readers to choose methods for their investigations.

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Microbial plant pathogens causing qualitative and quantitative losses in all corps are present not only in the infected plants, but also in the environmental comprising of soil, water and air. The vectors present in the environment spread the microbial pathogens to short and/or long distances. Detection of microbial pathogens rapidly and reliably by employing suitable sensitive applicable for different ecosystems. The pathogens have to be identified precisely and differentiated and quantified to plan appropriate short- and long-term strategies to contain the incidence and spread of diseases induced by them. This book aims to present all relevant and latest information on the detection techniques based on the biological, biochemical, immunological and nucleic acid characteristics of microbial pathogens presents in the host plants, as well as in the natural substrates that support the survival and perpetuation of the pathogens.

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Volume 2
Chapter 1 Introduction
1.1. Microbial plant pathogens as a major limiting factor of crop production
1.2. Discovery of bacteria as plant pathogens
1.3. Detection of bacterial and phytoplasmal pathogens and disease diagnosis
References
Chapter 2 Detection of Bacterial and Phytoplasmal Pathogens in Plants
2.1 Detection of bacterial pathogens in plant organs
2.1.1 Biological methods
2.1.2 Biochemical methods
2.1.3 Immunoassays
2.1.4 Nucleic acid-based techniques
2.2 Detection of phytoplasmal pathogens in plant organs
2.2.1 Histochemical methods
2.2.2 Immunoassays
2.2.3 Nucleic acid-based techniques
2.3 Detection of bacterial pathogens in seeds and planting materials
2.3.1 Detection of bacterial pathogens in seeds
2.3.2 Detection of bacterial pathogens in planting materials
2.3.3 Detection of bacterial pathogens in postharvest produce
2.4 Detection of phytoplasmal pathogens in planting materials
Appendix
References
3. Detection of Bacterial and Phytoplasmal Pathogens in the Environment
3.1 Detection of bacterial pathogens in environment
3.1.1 Detection of bacterial pathogens in soil
3.1.2 Detection of bacterial pathogens in water
3.1.3 Detection of bacterial pathogens in air
3.1.4 Detection of bacterial pathogens in alternative host plants
3.1.5 Detection of bacterial pathogens in vector insects
3.2 Detection of phytoplasmal pathogens in environment
3.2.1 Detection of phytoplasmal pathogens in alternative host plants
3.2.2 Detection of phytoplasma pathogens in insect vectors
Appendix
References
Chapter 4 Assessment of Variability in Bacterial and Phytoplasmal Plant Pathogens
4.1 Assessment of variability in bacterial pathogens
4.1.1 Assessment of variations in pathogenicity
4.1.2 Assessment of variations in phage sensitivity
4.1.3 Assessment of variations in cultural characteristics
4.1.4 Assessment of variations in biochemical characteristics
4.1.5 Assessment of variations in immunological characteristics
4.1.6 Assessment of variations in genomic nucleic acid characteristics
4.1.7 Assessment of variations in resistance to chemicals
4.2 Assessment of variability in phytoplasmal pathogens
4.2.1 Assessment of variations in immunological characteristics
4.2.2 Assessment of variations in genomic nucleic acid characteristics
Appendix
References
Chapter 5 Diagnosis of Bacterial and Phytoplasmal Diseases
5.1 Choice of diagnostic tests for bacterial diseases
5.1.1 Conventional techniques
5.1.2 Biochemical tests
5.1.3 Immunoassays
5.1.4 Nucleic acid-based techniques
5.2 Choice of diagnostic tests for phytoplasmal diseases
5.2.1 Biological methods
5.2.2 Nucleic acid-based techniques
5.3 Agencies involved in disease diagnosis
5.3.1 Disease diagnostic centers
5.3.2 Plant quarantines
References

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